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KMID : 0358319960370111215
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Korean Journal of Urology
1996 Volume.37 No. 11 p.1215 ~ p.1220
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Contractile Responses of Rat Seminal Vesicle to Field Stimulation, Phenylephrine, Acethlcholine and KCI in Anoxia
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Abstract
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Oxygen supply is considered to one of the most important local factors influencing the contractility of smooth muscle. However, the effect of anoxia on the contractility is not uniform throughout the organs containing smooth muscle.
This study was undertaken to evaluate the changes of the contractility of rat seminal vesicle to field stimulation, phenylephrine (Phe), acetylcholine (Ach), and KC1 in anoxia. Seminal vesicle was rapidly removed from Sprague-Dawley rats which
had
been
killed by cervical dislocation. Spiral segments were mounted in organ bath containing Tyrode's solution at 37℃and equilibrated with 95% O2 and 5% CO2 for 1 hr. Anoxia was produced by changing the gas mixture to 95% N2, 5% CO2. The effect of
anoxia
on
the contractile responses to field stimulation, Phe, Ach, and KC1 was determined at different times after the initiation of anoxia. @ES The results of this study can be summarized as follows:
@EN 1. Anoxia mduced a time-dependent decrease of the contractile responses to field stimulation (30 and 60 Hz).
2. Anoxia potentially inhibited the rhythmic contractions to Phe and Ach. However, the basal tension showed a transient relatxation followed by a contraction after 10 min.
3. In tonic contraction produced by KC1, anoxia caused a transient relaxation followed by a further contraction. however, this anoxic contraction was not inhibited by berapamil (10E-1 M).
This study demonstrated that anoxia may cause poor contractile responses to field stimulation and increased basal tension to Phe, Ach, and KC1. But anoxic contraction by KC1 was not inhibited by verapamil, which means that this anoxic contraction
of rat
seminal vesicle is not mediated by Ca++ influx.
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